Pdf antirainbow dyespecific antibodies as universal tools. Product information thermo scientific pageruler plus. The ladder is supplied in gel loading buffer and is readytouse. The prestained protein ladder 245 kda is a threecolor prestained protein standard which includes protein dye for convenient protein sample staining. Two orange reference bands at 70 kda and 25 kda and one green reference band at 10 kda highlight the bluestained protein ladder. Thermo scientific pageruler plus prestained protein ladder. The protein ladder is conveniently packaged and ready to. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. All four standards have the same gel migration patterns, with three highintensity reference bands 25, 50, and 75 kd. Relative rubisco content was performed using the method of li et al. We checked neural protein expression using this protein ladder as a marker for proteins of various molecular weights. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sds.
With, one of our best sellers, 3 tricolored prestained protein marker, you can load up over 300 wells for western blotting application. Three different chromophores are bound to the proteins, producing a brightly colored ladder see website for product images. In addition, the ladder proteins except for the 5kda peptide contain an integral streptag ii sequence and may be detected on western blots using streptactin conjugates. The pageruler unstained broad range protein ladder is supplied readytouse. Load an appropriate volume of the ladder on the gel. Thermo scientific pageruler prestained protein ladder is a mixture of ten 10 blue, orange and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Fig 7 pageruler prestained protein ladder thermo scientific ltd page 12 iii from bioc 46 at the university of hong kong. The protein ladder is supplied in a readytouse format for direct loading onto ge. Certificate of analysis pageruler plus prestained protein. The protein concentrations are carefully balanced for even intensity. Does pierce pageruler prestained protein ladder a mix of.
Pageruler prestained protein ladder, 10 to 180 kda. Thermo scientific pageruler unstained broad range protein. Product information thermo scientific pageruler unstained. The ladder is supplied in gel loading buffer and is ready to use.
Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 80 kda and 25 kda, respectively, that resolves into 12 sharp bands when electrophoresed. Mxstable prestained protein ladder is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kda. Mix the solution gently and thoroughly to ensure it is homogeneous. Thermo scientific pierce protein molecular weight marker. The thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. Pdf antirainbow dyespecific antibodies as universal. The color prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 72 kda and 26 kda, respectively, that resolves into 11 sharp bands when electrophoresed. Prestained protein ladders can have a sligtly different mobility and mass than the same peotein ladder which is not prestained. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels. Prestained protein ladder, broad range 10230 kda neb.
Pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sdspage and western blotting. The thermo scientific pageruler plus prestained protein ladders are a mixture of nine recombinant proteins ranging from 10 kda to 250 kda. Pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sdspage and. Use a bit less of the ladder 23 l for western blots. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. Proteins are covalently coupled with a blue chromophore except for two reference bands one green. Protein marker prestained protein ladders geneonbioscience.
The blueray prestained protein ladder is designed for monitoring protein separated during sdspolyacrylamide gel electrophoresis, verification of western transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximate sizing of proteins. Introducing thermo scientific pierce prestained and. Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda following electrophoresis. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. The protein ladder consists of 12 prestained proteins covering a wide range of molecular weights 10 to 245 kda. However, an unstained protein ladder can only be visualized following staining with coomassie, or a similar nonspecific protein stain.
Bluestain protein ladder, 11245 kda goldbio asset 1. The pageruler plus prestained protein ladder is a mixture of 9 recombinant, highly purified colored proteins with apparent molecular weights of 10 to 250 kda. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sds. Fig 7 pageruler prestained protein ladder thermo scientific. Prestained protein standards life science research biorad.
Procedure for using the protein ladder in polyacrylamide gel electrophoresis 1. The molecular mass of protein bands were estimated by comparison with an established protein marker cleaver scientific lda. The apparent molecular weight of the prestained proteins are given in the table below. Product insert cozy prestained protein ladder the ladder is ready for loading. Product information thermo scientific pageruler unstained protein.
Blueray prestained protein ladder focus bioscience. Hxstable prestained protein ladder dna protein ladders. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage and western blotting. Opal prestained protein standard 10180kda mb2090500. The thermo scientific pageruler unstained protein ladder consists of a unstained mixture of 14 recombinant, highly purified proteins ranging from 10 kda to 200 kda. Benchmark prestained protein ladder must show 10 major bands when separated by electrophoresis on a novex 420% trisglycine gel. Prestainded protein ladder with 10 bands an a range from 6,5 kd to 270 kd protein marker extended ps. Thermo scientific pageruler prestained protein ladder, 10.
Page 2 directions the benchmark prestained protein ladder is supplied in a readytouse format. The protein ladder is conveniently packaged and ready to use with no heating. Pink plus prestained protein ladder 10175kda cleaver. Take 35 l of the ladder for a small size gel slot for electrophoresis. Precision plus protein prestained standards are available in dual color, all blue, kaleidoscope, and dual xtra options. Other proteins are coupled with a blue chromophore. Color prestained protein standard, broad range 10250 kda. Unstained versus prestained protein ladders scientist solutions. Thermo scientific pageruler prestained protein ladders the thermo scientific pageruler and pageruler plus prestained protein ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. Protein molecular weight marker ps10 prestained is ideal for precise sizing of proteins by sdspage and for western blotting.
The protein mw markers in this ladder resolve into sharp bands. It is prestained and has multicoloured peptides which makes it easy to identify. Page 4 benchmark prestained protein ladder limited use. Prestained protein ladder, broad range 10230 kda is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. It contains two orange proteins as reference bands. Prestained protein ladders and unstained protein ladders abcam. Antirainbow dyespecific antibodies as universal tools for the visualization of prestained protein molecular weight markers in western blot analysis. The ladder is visualized by sdspage using coomassie or silver stains or detected in western blots with protein stains. The bluelf prestained protein ladder is designed for monitoring protein separation during sdspage, verification of western transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximating the size of p roteins. Page 3 example the apparent molecular weights of the protein bands in benchmark prestained protein ladder are shown below. Mixes very good with about why the right fermentas pageruler protein ladder sm eating plan works list in its category for more than a year.
The protein ladder is supplied in a readytouse format for direct loading. Five microliters of benchmark prestained protein ladder was separated on a. Monitor protein migrationsample in sdspage, monitor protein transfer onto membranes during western blotting, sizing of proteins on sdspage and western blots introduction prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. The hxstable prestained protein ladder is a threecolor protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kda. Thermo scientific pageruler prestained protein ladder, 10 to 180 kda mixture of 10 blue, orange and greenstained proteins 10 to 180kda used as size standards in protein electrophoresis sdspage and western blotting. Mxstable prestained protein ladder dna protein ladders. The blueray prestained protein ladder is designed for monitoring protein separated during sdspage, verification of western transfer efficiency on membranes pvdf, nylon or nitrocellulose and for approximate sizing of proteins. Opal prestained protein standard 10180kda is designed for monitoring protein separated during sdspolyacrylamide gel electrophoresis, verification of western blot transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximate sizing of proteins. Does pierce pageruler prestained protein ladder a mix of phosphorylated protein. The readytouse bluestain protein ladder covers a molecular weight between 24510 kda, and is used for protein electrophoresis operations. Protein ladder with bands and broad range from 5 245 kda protein ladder ps10 plus.
Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Thermo scientific pageruler prestained nir protein ladder the pageruler prestained nir protein ladder is a mixture of 10 proteins 11 to 250kda that are bluestained and fluorlabeled for nearir fluorescent visualization and protein sizing. The thermo scientific pageruler prestained protein ladder is a prestained mixture of ten recombinant proteins ranging from 10k to 180k. Page 4 benchmark prestained protein ladder limited use label. Cst prestained protein marker, broad range 11190 kda. Pageruler plus prestained protein ladder, 10 to 250 kda. Prestained protein marker, broad range 7175 kda neb.
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